Coding

Part:BBa_K2088011:Design

Designed by: Shi Kejian   Group: iGEM16_NAU-CHINA   (2016-10-08)


MhbR: a protein that combines the 3HBA to induce the promoter Mhbpt.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 433
    Illegal AgeI site found at 516
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 738


Design Notes

None


Source

The gene of MhbR can be gotten from the bacteria M5a1 using PCR.The sequence of the gene comes from GenBank https://www.ncbi.nlm.nih.gov/nuccore/EU165545.1

References